Neural Engineering

Transformative Technologies

Work Package: WP5
Programme: P12
Deliverable: 12.3 Demonstration of platform for combined two-photon targeted robotic patch clamping

Deliverable due date: month 36.

This document reports on the progress of work on Deliverable 12.3. In project P12, the ESR11 has been working on the development of a two-photon targeted patch-clamping robot; this is a specific instance of optogenetic BCI technology with significant prospects for high throughput in vivo drug characterization as well as reverse engineering neural circuits. It is a deviation from the initial project plan, since by the start of NETT, Imperial had already completed the tasks associated with the project. Thus a new, but related, project was commenced to replace P12. This builds on the original P12, by adding the capacity to target multiple individual glass recording pipettes onto visualised neurons
in vivo, in order to stimulate action potentials by passing current into them, to control the membrane potential of neurons, or to monitor their membrane potential. This provides a “gold standard” single neuron interfacing technology.
The deliverable 12.3 has been changed to: “Demonstration of robotically automated whole cell patch clamp recording in vivo”. All the planned tasks related to this deliverable have been accomplished. We will describe our demonstration of a combined two-photon targeted robotic patch clamping in vivo. Obtained results will be presented in national and international conferences and published in high impact factor journals (in preparation).
The work also covers milestone 4.

Contributors: Luca Annecchino, Simon Schultz (Imperial)